The Effect of Branched-Chained Amino Acids on GLUT1 protein in the Bovine Mammary Epithelial Cells in the Presence and Absence of Glucose.

Nadiia Stepanchenko

This semester I got opportunity to continue my work with Dr. Ranga Appuhamy on a different but related to the old one project. Last semester my task was to see if branched-chain amino acids impact the abundance of the glucose transporter protein 1(the most common protein transporter that also drives the milk volume in cattle) in the mammary epithelial cell of the cow (these cells are responsible for milk production in the cow). During that semester we found out that taking away the branched-chain amino acids also decreases the amount of the glucose transporter protein 1 in the cell. This semester we decided to look into a couple different things. First, we wanted to see if the absence or presence of glucose in the treatment media (substance in which the cells are growing) will change the result (last semester we had glucose and insulin available to the cell in the media). Secondly, we wanted to look into the cell signaling that creates that change (essentially which protein tells the cell that it is time to bring more glucose in).

Effect of Amino Acids PosterWorking in the lab

 

 

 

 

 

 

 

 

 

 

 

 

 

Duration: 04/30/2019

Principal Investigator(s): Ranga Appuhamy